Séminaire de Norbert BAKALARA
invité par Guy LENAERS
Le 26 février 2016
Un séminaire sera donné par Norbert BAKALARA, Institut des Neurosciences de Montpellier.
«Phostine: a new class of glycomimetic targeting glycosylation in cancer cells»
Les étudiants souhaitant assister à la conférence doivent s'inscrire au préalable avant le jeudi 25 février 12h00 dans la limite des places disponibles auprès de : secr-inserm-angers @ contact.univ-angers.fr en communiquant leur numéro de carte étudiante.
Pour que la participation au séminaire soit validante, il est impératif que les étudiants émargent la feuille de présence.
Pour rappel, les étudiants non inscrits ne seront pas acceptés.
12h00 - 13h00
CHU Angers - Bâtiment IBS-IRIS – Salle de conférence RDC
secr-inserm-angers @ contact.univ-angers.fr
Tumor progression is correlated with the expression of N-glycans bearing tri- and tetra- antennary β1,6-N-acetylglucosamine. Glioblastoma were reported to express highly variable amounts of both GnT-III and GnT-V mRNA suggesting an important physiological role in maintaining the balance of these exclusive enzymatic activities. Phostine PST3.1a alters β1,6-GlcNAc and the β1,4-GlcNAc N-glycans of Glioblastoma Stem cells (GBMSC) neurospheres, by inhibiting the GnT-V enzymatic activity and modifying GnT-III expressions. In vitro, PST3.1a inhibits the stemness character of GBMSC by altering cell/cell, cell/neurosphere and neurosphere/neurosphere interactions. PST3.1a also inhibits the invasion and migration capacities of GBMSC. Orthotopic models of GBMSC grafts show reduction of more than 90% of their invasive and proliferative capacities. Glioma-specific GnT-V enzymatic activity revealed by the L-PHA lectin is affected in the brains of treated animals and galectin-1 expression is specifically inhibited. Finally, David analysis performed on the NCI-60 data indicates that PST3.1a selective cytotoxic activity against a sub-panel of cancer cell lines is negatively correlated with the expression of mitochondrial genes suggesting a link between the Hexose Biosynthetic Pathway and the Energetic Metabolism.